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  Home > JCE Print > Journal of Chemical Education > Issues > 1997  > January  >
High-Performance Liquid Chromatography
Laboratory Applications
HPLC for Undergraduate Introductory Laboratories
Scott A. Van Arman and Marcus W. Thomsen
Department of Chemistry, Franklin and Marshall College, Lancaster, PA 17604
Cover
January 1997
Vol. 74 No. 1
p. 49
Abstract
Undergraduate laboratories continue increasing the use of instrumentation in teaching. One technique that is growing in popularity is HPLC. We have designed a set of simple HPLC separations as part of an introductory set of projects that serve as an introduction to chromatography early in the organic course. We have introduced quantitative analysis to the common separation of analgesics so that students may identify the composition of an unknown commercial tablet. Derived from this system is an adaptation of the well known separation of nucleosides by reversed-phase HPLC such that students can quantitatively identify the components of an unknown "RNA digest." Students must determine retention times and an instrumental response factor for each component. For both separations all components elute in × 6 min. and baseline separation is excellent. From the retention times of standard individual component samples the identity of each component in the sample can be ascertained. From the instrumental response factors of standard individual component samples the percent composition of each component can be calculated.
More Information
*  Citation
 Van Arman, Scott A. and Thomsen, Marcus W. J. Chem. Educ. 1997 74 49.
*  Keywords
 Organic Chemistry, Laboratory Instruction, Chromatography, Nucleosides/Nucleotides, Quantitative Analysis
*  History
 Created:
Last Updated:		 July 29, 1999
June 23, 2005
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