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  Home > JCE Print > Journal of Chemical Education > Issues > 1998  > October  >
In the Laboratory
A Simplified Method for the Microscale Extraction of Pigments from Spinach
Kimberley R. Cousins and Kathleen M. Pierson
Department of Chemistry, California State University, San Bernardino, San Bernardino, CA 92407
Cover
October 1998
Vol. 75 No. 10
p. 1268
Abstract
A method is presented for microscale sample preparation for the thin-layer chromatographic analysis of the pigments in spinach. A commercial vegetable juicer is used for the initial extraction from spinach. This is followed by filtration, liquid/liquid extraction, centrifugation, and evaporation. The new sample preparation technique is significantly more efficient than present methods and minimizes solvents used for extraction.
More Information
*  Citation
 Cousins, Kimberley R.; Pierson, Kathleen M. J. Chem. Educ. 1998 75 1268.
*  Keywords
 laboratory instruction, chromatography, microscale, plant chem
*  History
 Created:
Last Updated:		 June 21, 1999
June 23, 2005
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