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| Home > JCE Print > Journal of Chemical Education > Issues >
2000
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October
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In the Laboratory
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Candies to Dye for: Cooperative, Open-Ended Student Activities to Promote Understanding of Electrophoretic Fractionation
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Randall Emry
Southeast High School, 2930 S. 37th St., Lincoln, NE 68506
Robert D. Curtright
Northeast High School, 2635 N. 63rd St., Lincoln, NE 68507
Jonathan Wright and John Markwell
Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0664
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October 2000
Vol. 77 No. 10
p. 1323
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| Abstract |
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A three-part series of laboratory activities is presented that allows students to learn why electrophoretic separations work and to manipulate the factors that influence the separation process. In the first two exercises, students perform cooperative separations of FD&C dyes from candies utilizing electrophoresis in two buffers of different pH. Students must use package information from one brand of candy and critical thinking to determine the identities of these dyes. Using structural formulas for the dyes the students draw conclusions regarding the effect of charge and molecular mass on separation. The final activity is an open-ended investigation into a student-formulated question. These activities integrate topics in biology and chemistry into activities that interest students and foster a real understanding of the tool of electrophoresis.
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| Supplement |
Student handouts and instructor notes are available as supplemental material.
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 Contents |
JCE2000p1323Wfigures.doc (Microsoft Word 98, Macintosh), JCE2000p1323Wstudent.doc (Microsoft Word 98, Macintosh), JCE2000p1323Wteacher.doc (Microsoft Word 98, Macintosh), F0190878.pic (Photoshop PICT, Macintosh), F0290878.cdr (ChemDraw), F0390878.cdr (ChemDraw), F0490878.pic (Photoshop PICT, Macintosh)
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JCE2000p1323W.pdf
JCE2000p1323W.sit
JCE2000p1323W.zip |
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| More Information |
Citation |
Emry, Randall; Curtright, Robert D.; Wright, Jonathan; Markwell, John. J. Chem. Educ. 2000 77 1323.
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Keywords |
Biochemistry; Dyes; Electrophoresis; Laboratory Instruction; Separation Science
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History |
Created:
Last Updated: |
September 22, 2000
April 15, 2005
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| Home > JCE Print > Journal of Chemical Education > Issues >
2000
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