Plant Pigments in Spinach. Three thin-layer chromatography plates show the separation of plant pigments in various spinach extracts. The separated pigments are xanthophyll, chlorophyll a and b, pheophytin a and b, and &beta-carotene. A direct method of extracting plant pigments from spinach leaves into a dry organic solvent and the subsequent chromatographic separation of the pigments is described in the article "An Improved Method for the Extraction and Thin-Layer Chromatography of Chlorophyll a and b from Spinach" by Hao T. Quach, Robert L. Steeper, and G. William Griffin.
Spinach image courtesy of LSU Agricultural Center.

The cover was designed by Betsy True.
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