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  Home > JCE Print > Journal of Chemical Education > Issues > 2006  > May  >
In the Classroom
JCE DigiDemos: Tested Demonstrations
Classroom Demonstrations of Concepts in Molecular Fluorescence
Jonathan P. Blitz, Daniel J. Sheeran, and Thomas L. Becker
Department of Chemistry, Eastern Illinois University, Charleston, IL 61920-3099

checked by: Neil D. Danielson
Department of Chemistry and Biochemistry, Miami University, Oxford, OH 45056

Cover
May 2006
Vol. 83 No. 5
p. 758
Abstract
A multichannel CCD-array spectrophotometer and handheld UV lamp are utilized to acquire and project in real-time the fluorescence emission spectra of quinine, fluorescein, eosin yellow, and rhodamine B. Instrumental concepts of fluorescence are illustrated based on the setup of the demonstration. The emission spectra of the fluorophores are correlated with the visual color of the luminescence. The absorbance and fluorescence emission spectra of quinine in tonic water, along with the Hg source spectrum, are visually compared. This demonstration provides a framework for describing the transitions involved in fluorescence spectroscopy.
More Information
*  Citation
 Blitz, Jonathan P.; Sheeran, Daniel J.; Becker, Thomas L. J. Chem. Educ. 2006 83 758.
*  Keywords
 Analytical Chemistry; Demonstrations; Dyes / Pigments; First-Year Undergraduate / General; Fluorescence Spectroscopy; Instrumental Methods; Second-Year Undergraduate; Upper-Division Undergraduate; UV-Vis Spectroscopy
*  History
 Created:
Last Updated:		 3/16/2006
3/22/2006
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