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2007
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In the Laboratory
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Cost-Effective Teacher
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Fluorescence Spectroscopy in a Shoebox
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M. Farooq Wahab
Department of Chemistry, University of Karachi, Karachi 75270, Pakistan
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August 2007
Vol. 84 No. 8
p. 1308
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| Abstract |
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This article describes construction of a simple, inexpensive fluorometer. It utilizes a flashlight or sunlight source, highlighter marker ink, bowl of water with mirror as dispersing element, and colored cellophane sheets as filters. The human eye is used as a detector. This apparatus is used to demonstrate important concepts related to fluorescence spectroscopy. Using ink from a highlighter marker, one can demonstrate the difference between light scattering and fluorescence emission, the need for an intense light source, phenomenon of the Stokes shift, the choice of filters, the preferred geometry of excitation source and emission detector, and the low detection limits that can be achieved by fluorescence measurements. By reflecting the fluorescence emission from a compact disk, it can be seen that the light emitted by molecules is not monochromatic. Furthermore, a spectrofluorometer is constructed using gratings made from a DVD or a CD. The shoebox fluorometer and spectrofluorometer can serve as useful teaching aids in places where commercial instruments are not available, and it avoids the black box problem of modern instruments.
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| More Information |
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Citation |
Wahab, M. Farooq. J. Chem. Educ. 2007, 84, 1308.
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Keywords |
Analytical Chemistry; Dyes / Pigments; First-Year Undergraduate / General; Fluorescence Spectroscopy; Hands-On Learning / Manipulatives; High School / Introductory Chemistry; Inquiry-Based / Discovery Learning; Laboratory Equipment / Apparatus; Laboratory Instruction; Qualitative Analysis; Spectroscopy
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History |
Created:
Last Updated: |
6/22/2007
7/5/2007
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 Caution! | | Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations. |
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