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2007
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In the Laboratory
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A Colorimetric Analysis Experiment Not Requiring a Spectrophotometer: Quantitative Determination of Albumin in Powdered Egg White
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A High School or General Science Experiment
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Amanda K. Charlton, Richard S. Sevcik, Dorie A. Tucker, and Linda D. Schultz
Department of Chemistry, Geosciences, and Environmental Science, Tarleton State University, Stephenville, TX 76402
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May 2007
Vol. 84 No. 5
p. 826
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| Abstract |
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One of the most widely used methods in modern chemical analysis is colorimetric analysis, which relies on formation of a color by the analyte. The intensity of this color is then usually measured using a spectrophotometer. Unfortunately, the cost of a spectrophotometer is often prohibitive for smaller schools, so many secondary school students are not exposed to these techniques. other common limitations for high school chemistry laboratory experiments include cost of chemicals, scientific glassware, and problems with disposal of chemical wastes. Albumin in powdered egg white was reacted with bromophenol blue in a pH 4.0 acetate buffer solution. The resulting blue color was compared to that formed by an albumin standard (1.00 g/500 mL) using a visual comparator method similar to that developed by Duboscq. The procedure is simple, rapid, accurate, inexpensive, and produced no hazardous wastes.
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| Supplement |
Student instructions, notes for the instructor, safety and hazards, and sample data are available.
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| More Information |
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Citation |
Charlton, Amanda K.; Sevcik, Richard S.; Tucker, Dorie A.; Schultz, Linda D. J. Chem. Educ. 2007, 84, 826.
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Keywords |
Analytical Chemistry; Bioanalytical Chemistry; Dyes / Pigments; Hands-On Learning / Manipulatives; High School / Introductory Chemistry; Laboratory Instruction; Proteins / Peptides; Quantitative Analysis
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History |
Created:
Last Updated: |
3/22/2007
4/9/2007
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 Caution! | | Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations. |
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