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In the Laboratory
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A Qualitative Experiment To Analyze Microbial Activity in Topsoil Using Paper and a Handmade Reflection Photometer
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Julius Kofi Agbeko
Teacher Education Division, Accra Teacher Training College, Accra, Ghana
Masakazu Kita
Faculty of Education, Okayama University, Okayama, Japan
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October 2007
Vol. 84 No. 10
p. 1689
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| Abstract |
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This article describes a novel, hands-on method to qualitatively determine the extent of microbial activity in topsoil using ordinary blank paper. Appropriate and scalable for the high school and college level, these experiments expose students to some of the challenges facing environmental researchers and also contribute to curriculum development of soil topics for environmental education. Through experimentation, students discover that paper containing high amounts of starch can be used to determine the degree of microbial activity in topsoil. After the application of soil solution eluent to paper, the paper is allowed to dry. Students react the paper with a KI + I2 solution, and a deep blue coloration results that diminishes over time. Using the principles of complementary color combinations and a simple, handmade, cadmium sulfide–light emitting diode (CdS–LED) reflection photometer, the color can be measured and the extent of microbial activity established.
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| Supplement |
A number of materials related to this paper are available. They include: a longer version of the paper with additional figures, a table, and directions for constructing the reflection photometer; notes for the instructor; and instructions for students.
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| More Information |
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Citation |
Agbeko, Julius Kofi; Kita, Masakazu. J. Chem. Educ. 2007, 84, 1689.
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Keywords |
Analytical Chemistry; Bioanalytical Chemistry; Environmental Chemistry; First-Year Undergraduate / General; Hands-On Learning / Manipulatives; High School / Introductory Chemistry; Inquiry-Based / Discovery Learning; Laboratory Equipment / Apparatus; Laboratory Instruction; Quantitative Analysis; Spectroscopy
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History |
Created:
Last Updated: |
8/20/2007
9/7/2007
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 Caution! | | Experiments, laboratory exercises, lecture demonstrations, and other descriptions of the use of chemicals, apparatus, instruments, computers, and computer interfaces are presented in the Journal of Chemical Education as illustrative of new or improved ideas or concepts in chemistry instruction and are directed at qualified teachers. Although every effort is made to assure and encourage safe practices and safe use of chemicals, the Journal of Chemical Education cannot assume responsibility for uses made of its published materials. Many chemicals are hazardous. Precautions for the safe use of hazardous chemicals and directions for their proper disposal are described in the Material Safety Data Sheets and on the labels. We strongly urge all those planning to use materials from our pages to make choices and to develop procedures for laboratory and classroom safety in accordance with local needs and situations. |
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