Removing Supernatant and Washing Precipitate

CCA! Volume 6

Home

Table of Contents

Keyword Index

About CCA!

Removing Supernatant and Washing Precipitate

After separating in a centrifuge, the supernatant liquid can be removed from the solid.

Decant the Supernatant

View Movie
.5 MB
15 Seconds

View Slides
4 Slide(s)

Remove the supernatant by pouring.

Removing the Supernatant with a Dropper

View Movie
.2 MB
8 Seconds

View Slides
3 Slide(s)

Remove the supernatant with a pipet or dropper.

Error in Removing the Supernatant with a Dropper

View Movie
.3 MB
10 Seconds

View Slides
4 Slide(s)

If the tip of the medicine dropper comes near the solid some of the solid may be removed with the supernatant.

Rinsing the Solid

View Movie
.7 MB
23 Seconds

View Slides
6 Slide(s)

The same sample is washed three times. Initially, the supernatant is very blue due to the impurities in the sample. With successive washings, the supernatant becomes less colored as the impurities are removed.

Rinse Solid and Re-Centrifuge (long version)

View Movie
2 MB
83 Seconds

View Slides
11 Slide(s)

Solid is rinsed and centrifuged.

Rinse Solid and Re-Centrifuge (short version)

View Movie
.8 MB
36 Seconds

View Slides
6 Slide(s)

Solid is rinsed and centrifuged.