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| Volume 1B Number 2 | ||
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| In This Issue | ||
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| HPLC: A Computer Simulation of High-Performance Liquid Chromatography | ||
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Robert C. Rittenhouse
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| About This Issue | ||
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John W. Moore University of Wisconsin-Madison, Madison, WI 53706-1396 |
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Note: |
This issue is out of print. A Windows version of HPLC is available. Please see issue 3D, 2 for more information. |
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The single program in this issue, HPLC, is one of only a few instrument simulator programs currently extant (1, 2). As a flight simulator provides an introduction to the situations a pilot will encounter, so an instrument simulator provides a very realistic introduction to the operation of a scientific instrument. Students can easily and quickly practice making the decisions that will need to be made when they operate a real instrument, and a much broader range of chromatographic separations becomes available to them than would be possible with a real instrument. The pedagogic advantages of using the simulator both before and after students operate the real instrument are enormous. Even if only a few computers are available, the much greater speed with which simulated separations can be carried out allows much more learning to occur. This program is a much expanded version of one that has already appeared in the Project SERAPHIM Catalog (3). In particular, the computer data system is much improved--so much so that it compares favorably with most of the commercial instruments available. Also, the detector has been enhanced to include a diode-array mode, which permits the complete spectrum of any of the chromatographic peaks to be recorded and displayed. Hardware and Software Requirements HPLC runs under MS-DOS or IBM PC DOS and is supplied on a 5.25-in. disk. It can be used on a computer with either a single 5.25-in. or a single 3.5-in. floppy disk drive, or on a computer with a hard disk. At least 512K of RAM is required as is a graphics adapter (CGA, EGA, or VGA). In some cases one may wish to have a graphics printer to print copies of the chromatograms, but the program will run without a printer. HPLC requires a mouse, since most choices are made by moving a mouse cursor to one or another screen graphic that represents one or another module of the instrument. The mouse must have mouse-driver software that is compatible with the Microsoft mouse. Such software is supplied with most mice; it is not included with the HPLC disk. Literature Cited |
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Last Updated: March 19, 2001 Created: December 10, 1996 |
Created by: J.L. Holmes Comments to: jceonline@chem.wisc.edu |
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| © 1997 Division of Chemical Education, Inc., American Chemical Society. All rights reserved. | |