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  Home > JCE Print > Journal of Chemical Education > Issues > 1995  > August  >
Laboratory Experiments
A Fast and Inexpensive Western Blot Experiment for the Undergraduate Laboratory
Shawn O. Farrell and Lynn E. Farrell
Colorado State University, Ft. Collins, CO
Cover
August 1995
Vol. 72 No. 8
p. 740
Abstract
Western blotting is an important, modern technique for transferring proteins from a gel onto nitrocellulose or other suitable support and then detecting a protein of interest using antibodies. We have developed an experiment and optimized the conditions for the undergraduate laboratory. The experiment can be done quickly using an electrophoretic blotter or more cheaply using passive transfer. This experiment allows the student to learn valuable procedures currently used in biochemistry and other biological sciences.
More Information
*  Citation
 Farrell, Shawn O.; Farrell, Lynn E. J. Chem. Educ. 1995 72 740.
*  Keywords
*  History
 Created:
Last Updated:		 10/1/1999
5/22/2006
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