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  Home > JCE Print > Journal of Chemical Education > Issues > 1995  > December  >
Features
The Modern Student Laboratory
Using the Glucose Oxidase/Peroxidase System in Enzyme Kinetics
Robert C. Bateman, Jr. and Jeffrey A. Evans
Department of Chemistry and Biochemistry, University of Southern Mississippi, Hattiesburg, MS 39406
Cover
December 1995
Vol. 72 No. 12
p. A240

Abstract
An expeimental protocol is described for a biochemistry laboratory on enzyme kinetics using glucose oxidase coupled to a horseradish peroxidase/colored dye system to yield an easily visualized and quantitated product. The experiment is designed to illustrate the concepts of specific activity, substrate specificity and kinetic parameters. Optional extensions of the protocol could explore parameters of coupled enzyme systems, reversible inhibition and enzyme immobilization.
More Information
*  Citation
Bateman, Robert C., Jr.; Evans, Jeffrey A. J. Chem. Educ. 1995 72 A240.
*  Keywords
*  History
Created:
Last Updated:
10/1/1999
5/22/2006
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