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  Home > JCE Print > Journal of Chemical Education > Issues > 1995  > July  >
General Interest
An Integrated Approach to the Undergraduate Biochemistry Laboratory
James G. Harman, John A. Anderson, Richard A. Nakashima, and Robert W. Shaw
Texas Tech University, Lubbock, TX 79406
Cover
July 1995
Vol. 72 No. 7
p. 641
Abstract
Undergraduate biochemistry laboratories traditionally expose students to biochemical techniques through a series of independent and usually unrelated laboratory exercises. Efforts to reorganize and update the undergraduate biochemistry laboratory at Texas Tech University have centered upon the development of a series of laboratory experiments that focus on a single biological system, the complex 11 (succinate:ubiquinone oxidoreductase) of Escherichia coli. Students are provided a computer-aided research environment in which to gain practical training in molecular biology, protein purification and enzyme kinetics. The laboratory schedule includes exercises on the succinate dehydrogenase operon (sdh) DNA sequence, and experiments that deal with isolation and characterization of sdh operon DNA, extraction and purification of complex 11 and characterization of complex 11 subunit structure and kinetic parameters. This unified approach to the biochemistry teaching laboratory is specifically designed to impact undergraduate student preparation for future studies, providing exposure to fundamental techniques of biochemistry experimentation and simulating the focused, single system, environment of a research laboratory.
More Information
*  Citation
 Harman, James G.; Anderson, John A.; Nakashima, Richard A.; Shaw, Robert W. J. Chem. Educ. 1995 72 641.
*  Keywords
*  History
 Created:
Last Updated:		 10/1/1999
5/22/2006
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