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  Home > JCE Print > Journal of Chemical Education > Issues > 1995  > October  >
Laboratory Experiments
DNA Composition Analysis by Nuclease Digestion and HPLC
Steven M. Wietstock
Department of Biochemistry, Alma College, 614 W. Superior Street, Alma, MI 48801
Cover
October 1995
Vol. 72 No. 10
p. 950
Abstract
In the field of nucleic acid research, one physical property of genomic DNA that is of use to the biochemist is the base composition of the DNA expressed in terms of the cytosine and guanosine content. Over the years, the most common method utilized for the determination of the mole fraction of G+C is the melting temperature of the DNA as determined by a spectrophotometric method. A new method for the determination of the mole fraction of G+C has been developed that requires only a sample of heat denatured DNA, a buffered nuclease P1 solution, and a reverse phase isocratic high performance liquid chromatography system. This method is discussed in detail for this article. Results are obtained in two hours time, and the need for an extensive temperature-regulated spectroscopy system is eliminated.
More Information
*  Citation
 Wietstock, Steven M. J. Chem. Educ. 1995 72 950.
*  Keywords
*  History
 Created:
Last Updated:		 10/1/1999
5/22/2006
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