JCE Online Journal of Chemical EducationDivision of Chemical Education, American Chemical SocietyAmerican Chemical Society
 | Subscriptions  | Software Orders  | Support  | Contributors  | Advertisers  | 

JCE Print
   Current Issue
 Previous Issues
 Supplements
 Search JCE Index

JCE Digital Library
   ChemInfo
 DigiDemos
 Featured Molecules
 LivTexts
 LrnCom
 QBank
 SymMath
 WebWare

JCE Software
   Latest Releases
 Software & Video
 Downloads
 Support

Only@JCE Online
   JCE Online Store
 JCE HS CLIC
 JCE Discussion Forums
   Biographical Snapshots
 ChemEd Resource Shelf
 Featured Molecules
 Hal's Picks
 Project Chemlab
 Reviewed WWW Sites
 "Web-Ed" Articles

About JCE
   Features
 Publications
 Operations
 Outreach
 Contact Us


  Home > JCE Print > Journal of Chemical Education > Issues > 1996  > April  >
Laboratory Experiments
Bacterial Toxicity Assessment Using Resazurin Reduction Kinetics: An Undergraduate Toxicity Experiment for the Chemistry-Biology Major
S. Helliwell and T. J. Harden
School of Science and Technology, Charles Sturt University, Wagga Wagga, NSW, Australia 2678
Cover
April 1996
Vol. 73 No. 4
p. 368
Abstract
An undergraduate toxicity experiment is described using the redox indicator resazurin as an indicator of dehydrogenase activity. Two types of bacteria are utilized, the Gram positive Streptococcus cremoris and a Gram negative bacterium, Escherichia coli, to demonstrate the potentially different toxicological responses to the same chemical. The resazurin reduction is followed spectrophometrically and appears to follow zero order kinetics, with absorbance versus time plots giving correlation coefficients of 0.99 or better. Working in pairs, students are easily able to carry out 10 kinetic runs in a three hour laboratory session. Percent inhibition by a chemical is determined by comparison with controls. Toxicity data are presented for a series of chlorobenzenes and the Hg2+ ion for two bacteria.
More Information
*  Citation
 Helliwell, S.; Harden, T. J. J. Chem. Educ. 1996 73 368.
*  Keywords
*  History
 Created:
Last Updated:		 9/23/1999
5/22/2006
<< Previous Article Table of Contents Next Article >>
  Home > JCE Print > Journal of Chemical Education > Issues > 1996 > April > Page 368


Subscriptions
 Subscription Info
 Order Forms

JCE HS CLIC

Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.
 JCE HS CLIC

Contributions Welcome
JCE welcomes your submission
 Contributors' Corner

Advertisers
In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first.
 Recent Advertisers

Be An Ambassador
Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants.
 Outreach with JCE

Comments to
jceonline@chem.wisc.edu		 Copyright © Division of Chemical Education, Inc., American Chemical Society.
All rights reserved.