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  Home > JCE Print > Journal of Chemical Education > Issues > 1996  > August  >
Secondary School Chemistry
Filtrates and Residues
A Simulated Growth Hormone Analysis
Mary Harris
John Burroughs School, 755 South Price Road, St. Louis, MO 63124
Cover
August 1996
Vol. 73 No. 8
p. 735
Abstract
Growth hormone is a drug that is sometimes abused by amateur or professional athletes for performance-enhancement. This laboratory is a semimicroscale simulation analysis of a sample of "urine" to detect proteins of two very different molecular weights. Gel filtration uses a 10 mL disposable pipette packed with Sephadex. Students analyze the fractions from the filtration by comparing colors of the Brilliant Blue Coomassie Dye as it interacts with the proteins in the sample to a standard set of known concentration of protein with the dye. The simulated analysis of growth hormone is intended to be included in a unit on organic chemistry or in the second year of high school chemistry.
More Information
*  Citation
 Harris, Mary. J. Chem. Educ. 1996 73 735.
*  Keywords
*  History
 Created:
Last Updated:		 9/23/1999
5/22/2006
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