JCE Online Journal of Chemical EducationDivision of Chemical Education, American Chemical SocietyAmerican Chemical Society
 | Subscriptions  | Software Orders  | Support  | Contributors  | Advertisers  | 

JCE Print
   Current Issue
 Previous Issues
 Supplements
 Search JCE Index

JCE Digital Library
   ChemInfo
 DigiDemos
 Featured Molecules
 LivTexts
 LrnCom
 QBank
 SymMath
 WebWare

JCE Software
   Latest Releases
 Software & Video
 Downloads
 Support

Only@JCE Online
   JCE Online Store
 JCE HS CLIC
 JCE Discussion Forums
   Biographical Snapshots
 ChemEd Resource Shelf
 Featured Molecules
 Hal's Picks
 Project Chemlab
 Reviewed WWW Sites
 "Web-Ed" Articles

About JCE
   Features
 Publications
 Operations
 Outreach
 Contact Us


  Home > JCE Print > Journal of Chemical Education > Issues > 1996  > July  >
Features
The Modern Student Laboratory
The Purification of Horse Heart Cytochrome c by Ion Exchange Chromatography
Patricia Ann Mabrouk
Department of Chemistry, Northeastern University, Boston, MA 02115
Cover
July 1996
Vol. 73 No. 7
p. A149
Abstract
While enormous changes have occurred in the past decade in the field of analytical chemistry, the curriculum for undergraduate Quantitative Analysis has changed very little. This paper describes a highly successful experiential learning laboratory experiment for use in undergraduate Quantitative Analysis which capitalizes on current student interest in biotechnology. In the multi-week experiment, students investigate the preparative scale purification of horse cytochrome c by ion exchange chromatography using a two-wavelength UV-vis purity assay to characterize the chromatographed protein. The experiment emphasizes critical thinking and allows students to assimilate and apply the fundamental concepts underlying separations (dialysis; ion exchange), acid-base (buffers), UV-vis spectrophotometry, and redox (oxidants/reductants) chemistry. Remarkably, while most students consider the experiment to be extremely difficult, 55% rank this experiment as the best experiment in our current quantitative analysis laboratory curriculum.
More Information
*  Citation
 Patricia Ann Mabrouk. J. Chem. Educ. 1996 73 A149.
*  Keywords
*  History
 Created:
Last Updated:		 9/23/1999
5/22/2006
<< Previous Article Table of Contents Next Article >>
  Home > JCE Print > Journal of Chemical Education > Issues > 1996 > July > Page A149


Subscriptions
 Subscription Info
 Order Forms

JCE HS CLIC

Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.
 JCE HS CLIC

Contributions Welcome
JCE welcomes your submission
 Contributors' Corner

Advertisers
In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first.
 Recent Advertisers

Be An Ambassador
Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants.
 Outreach with JCE

Comments to
jceonline@chem.wisc.edu		 Copyright © Division of Chemical Education, Inc., American Chemical Society.
All rights reserved.