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  Home > JCE Print > Journal of Chemical Education > Issues > 1996  > March  >
Laboratory Experiments
Isolation and Purification of Calmodulin from Bovine Brain: A Biochemistry Experiment
Jeanne M. Buccigross, Jerry W. Hayes II, and Christa S. Metz
College of Mount St. Joseph, Cincinnati, OH 45233-1670
Cover
March 1996
Vol. 73 No. 3
p. 273
Abstract
This article describes a procedure for the purification of an enzyme from a readily available animal tissue. It allows for classical methods of enzyme purification to be examined by students. These methods include homogenization, centrifugation, isoelectric precipitation, and affinity chromatography. The experimental procedure is straightforward and most equipment is available at even small colleges and universities. Most teaching methods of protein isolation do not include an affinity chromatography step and the experiment benefits from the inclusion of this technique into the protocol.
More Information
*  Citation
 Buccigross, Jeanne M.; Hayes II, Jerry W.; Metz, Christa S. J. Chem. Educ. 1996 73 273.
*  Keywords
*  History
 Created:
Last Updated:		 9/23/1999
5/22/2006
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