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  Home > JCE Print > Journal of Chemical Education > Issues > 1996  > November  >
In the Laboratory
The Microscale Laboratory
Rapid Microscale Isolation and Purification of Yeast Alcohol Dehydrogenase Using Cibacron Blue Affinity Chromatography
Chad Morgan and Neil Moir
Cover
November 1996
Vol. 73 No. 11
p. 1040
Abstract
A rapid microscale procedure has been developed for the isolation and purification of yeast alcohol dehydrogenase. Glass beads are used for cytolysis, PEG precipitation for partial purification and a cibacron blue affinity column for the final step. A 27.5 fold purification can be achieved in 2 - 3 hours.
More Information
*  Citation
 Morgan, Chad; Moir, Neil. J. Chem. Educ. 1996 73 1040.
*  Keywords
 Biochemistry
*  History
 Created:
Last Updated:		 August 5, 1999
February 21, 2006
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