DNA Sequencing is a package of five programs that
instructs and tests students on the Maxam-Gilbert method
of sequencing DNA. It can be used as a self-instruction
unit, as an assignment, and as a testing vehicle. The package
is aimed at a university level chemistry, biochemistry,
or chemical-biology course, but advanced senior high
school students will also find it useful. The central feature of
the package randomly generates a nucleic acid fragment
and simulates the subsequent Maxam-Gilbert autoradiogram. Students
are then guided through the method of interpreting the autoradiogram.
Students require a prior, general knowledge of the DNA base structures
and of the principles of electrophoresis. Knowledge of the chemistry of
the cleavage reactions used in the method is helpful but not
necessary to use these programs.
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