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  Home > JCE Print > Journal of Chemical Education > Issues > 1997  > October  >
Information • Textbooks • Media • Resources
Disadvantages of Double Reciprocal Plots
R. Bruce Martin
University of Virginia, Chemistry Department, McCormick Road, Charlottesville, VA 22901
Cover
October 1997
Vol. 74 No. 10
p. 1238
Abstract
Because they involve grossly uneven weightings of points, the linear and formally similar double reciprocal Benesi-Hildebrand and Lineweaver-Burke plots should never be used to resolve equilibrium and enzyme kinetic results. In such plots weightings from one end to the other may vary as much as a thousand or more. More equal weightings of the experimental points may be obtained by utilizing the linear, single reciprocal Scott and Hanes plots. The most appropriate resolution is achieved by a non-linear least squares analysis with the most error prone observable expressed as a function of all other variables and parameters.
More Information
*  Citation
 Martin, R. Bruce. J. Chem. Educ. 1997 74 1238.
*  Keywords
 Analytical Chemistry, Biochemistry, Biophysical Chemistry, Enzymes, Equilibrium, and Kinetics
*  History
 Created:
Last Updated:		 July 27, 1999
June 23, 2005
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