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1998
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April
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In the Laboratory
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Teaching Protein Crystallization by the Gel Acupuncture Method
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Juan Manuel García-Ruiz, Abel Moreno, F. Otálora, D. Rondón, C. Viedma, and F. Zauscher
Laboratorio de Estudios Cristalográficos, Instituto Andaluz de Ciencias de la Tierra, CSIC-Universidad de Granada, Av. Fuentenueva s/n, 18002 Granada, Spain
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April 1998
Vol. 75 No. 4
p. 442
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| Abstract |
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This paper provides a detailed description of a simple method to obtain large protein single crystals inside glass capillaries. The method is based upon the properties of gels, which are used to hold capillaries containing the protein solution, and also to act as the mass transport medium for the precipitating agent. Recipes for a set of selected experiments bringing a hands-on experience on the crystallization of different soluble proteins are supplied. These experiments are inexpensive and straightforward enough for teaching at the undergraduate level that large biological macromolecules that are the gate our structural studies and drug design can be crystallized. Using simple equations accounting for the solubility of proteins and for the nucleation process, the experimental results are explained to provide a rational approach to the problem. In addition, because of the nature of the crystallization method which is based on diffusion-reactions systems, the student is introduced to the meaning of self-organization.
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| More Information |
 Citation |
Juan Manuel García-Ruiz, Abel Moreno, F. Otálora, D. Rondón, C. Viedma, and F. Zauscher. J. Chem. Educ. 1998 75 442.
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Keywords |
Proteins, Laboratory Instruction, Crystallography, Biochemistry
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History |
Created:
Last Updated: |
June 25, 1999
June 24, 2005
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| Home > JCE Print > Journal of Chemical Education > Issues >
1998
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April
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442
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