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  Home > JCE Print > Journal of Chemical Education > Issues > 1998  > August  >
In the Laboratory
Automated Protein Assay Using Flow Injection Analysis
Carrie A. C. Wolfe, Matthew R. Oates, and David S. Hage
Department of Chemistry, University of Nebraska, Lincoln, NE 68588-0304
Cover
August 1998
Vol. 75 No. 8
p. 1025
Abstract
The technique of flow injection analysis (FIA) is a common instrumental method used in detecting a variety of chemical and biological agents. This paper describes an undergraduate laboratory that uses FIA to perform a bicinchoninic acid (BCA) colorimetric assay for quantitating protein samples. The method requires less than 2 min per sample injection and gives a response over a broad range of protein concentrations. This method can be used in instrumental analysis labs to illustrate the principles and use of FIA, or as a means for introducing students to common methods employed in the analysis of biological agents.
More Information
*  Citation
 Wolfe, Carrie A. C.; Oates, Matthew R.; Hage, David S. J. Chem. Educ. 1998 75 1025.
*  Keywords
 Instrumental Methods, Quantitative Analysis, Proteins, UV-Vis Spectroscopy, Biotechnology, Analytical Chemistry
*  History
 Created:
Last Updated:		 June 22, 1999
June 24, 2005
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