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1998
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In the Laboratory
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Working with Enzymes - Where Is Lactose Digested? An Enzyme Assay for Nutritional Biochemistry Laboratories
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Sandi R. Pope, Tonya D. Tolleson, R. Jill Williams, Russell D. Underhill, and S. Todd Deal
Georgia Southern University, Department of Chemistry, Landrum Box 8064, Statesboro, GA 30460-8064
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June 1998
Vol. 75 No. 6
p. 761
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| Abstract |
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At Georgia Southern University, we offer a sophomore-level introductory biochemistry course that is aimed at nutrition and chemistry education majors. The laboratory portion of this course has long lacked an experimental introduction to enzymes. We have developed a simple enzyme assay utilizing lactase enzyme from crushed LactAid tablets and a 5% lactose solution ("synthetic milk"). In the experiment, the students assay the activity of the enzyme on the "synthetic milk" at pHs of approximately 1, 6, and 8 with the stated goal of determining where lactose functions in the digestive tract. The activity of the lactase may be followed chromatographically or spectrophotometrically. The experiment, which is actually a simple pH assay, is easily implemented in allied health chemistry laboratory courses and readily lends itself to adaptation for more complex kinetic assays in upper-level biochemistry laboratory courses. The experimental details, including a list of required supplies and hints for implementation, are provided.
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Citation |
Pope, Sandi R.; Tolleson, Tonya D.; Williams, R. Jill; Underhill, Russell D.; Deal, S. Todd. J. Chem. Educ. 1998 75 761.
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Keywords |
Enzymes, Carbohydrates, Laboratory Instruction, Biochemistry
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History |
Created:
Last Updated: |
June 23, 1999
November 22, 2005
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| Home > JCE Print > Journal of Chemical Education > Issues >
1998
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