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  Home > JCE Print > Journal of Chemical Education > Issues > 1998  > October  >
In the Laboratory
Enzyme-Linked Antibodies: A Laboratory Introduction to the ELISA
Gretchen L. Anderson and Leo A. McNellis
Department of Chemistry, Indiana University South Bend, South Bend, IN 46634
Cover
October 1998
Vol. 75 No. 10
p. 1275
Abstract
A fast and economical laboratory exercise is presented that qualitatively demonstrates the power of enzyme-linked antibodies to detect a specific antigen. Although ELISAs are commonly used in disease diagnosis in clinical settings, this application uses biotin, covalently attached to albumin, to take advantage of readily available reagents and avoids problems associated with potentially pathogenic antigens. The laboratory exercise is suitable for high school or freshman level biochemistry courses, and can be completed within two hours.
More Information
*  Citation
 Anderson, Gretchen L.; McNellis, Leo A. J. Chem. Educ. 1998 75 1275.
*  Keywords
 enzymes, non-major courses, biochem, laboratory instruction, medicinal chem
*  History
 Created:
Last Updated:		 June 21, 1999
June 24, 2005
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