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  Home > JCE Print > Journal of Chemical Education > Issues > 1998  > October  >
In the Laboratory
Immobilized Lactase in the Biochemistry Laboratory
Matthew J. Allison and C. Larry Bering
Department of Chemistry, Clarion University, Clarion, PA 16214
Cover
October 1998
Vol. 75 No. 10
p. 1278
Abstract
Immobilized enzymes have many practical applications. They may be used in clinical, industrial, and biotechnological laboratories and in many clinical diagnostic kits. For educational purposes, use of immobilized enzymes can easily be taught at the undergraduate or even secondary level. We have developed an immobilized enzyme experiment that combines many practical techniques used in the biochemistry laboratory and fits within a three-hour time frame. In this experiment, lactase from over-the-counter tablets for patients with lactose intolerance is immobilized in polyacrylamide, which is then milled into small beads and placed into a chromatography column. A lactose solution is added to the column and the eluant is assayed using the glucose oxidase assay, available as a kit. We have determined the optimal conditions to give the greatest turnover of lactose while allowing the immobilized enzymes to be active for long periods at room temperature.
More Information
*  Citation
 Allison, Matthew J.; Bering, C. Larry. J. Chem. Educ. 1998 75 1278.
*  Keywords
 laboratory instruction, enzymes, biotechnology, biochem
*  History
 Created:
Last Updated:		 June 21, 1999
June 24, 2005
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