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  Home > JCE Print > Journal of Chemical Education > Issues > 1999  > December  >
In the Laboratory
A Brine Shrimp Bioassay for Measuring Toxicity and Remediation of Chemicals
Marya Lieberman
Department of Chemistry & Biochemistry, University of Notre Dame, Notre Dame, IN 46556
Cover
December 1999
Vol. 76 No. 12
p. 1689
Abstract
A bioassay using Artemia franciscana (brine shrimp) was adapted to measure the toxicity of household chemicals. One project is described in which students collect dose-response curves for seven commercial flea-killing products. Next, groups of students researched the insecticidal ingredients of the flea products. On the basis of the structures of the active ingredients, they chose remediation methods to make the flea product less toxic to brine shrimp; procedures included copper-catalyzed hydrolysis, adsorption onto activated charcoal, bleach treatment, and photodegradation. No special equipment or supplies are necessary for the bioassay other than the brine shrimp eggs, which can be obtained at any aquarium store.
Supplement
Chemical background for the experiment, detailed experimental materials and procedures, student handouts, instructions for preparing lab reports and group posters, a grading sheet for the posters, and further literature references are provided.
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*  Citation
 Lieberman, Marya. J. Chem. Educ. 1999 76 1689.
*  Keywords
 Laboratory Instruction; Safety / Hazardous Materials; Environmental Chemistry; Drugs / Pharmaceuticals; Medicinal Chemistry; Nonmajor Courses; Introductory / High School Chemistry
*  History
 Created:
Last Updated:		 November 10, 1999
November 22, 2005
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