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  Home > JCE Print > Journal of Chemical Education > Issues > 1999  > December  >
In the Laboratory
Chromatographic Separations Using Solid-Phase Extraction Cartridges: Separation of Wine Phenolics
Charles A. Brenneman and Susan E. Ebeler
Department of Viticulture & Enology, University of California, Davis, Davis, CA 95616
Cover
December 1999
Vol. 76 No. 12
p. 1710
Abstract
We describe a simple laboratory experiment that demonstrates the principles of chromatographic separation using solid-phase extraction columns and red wine. By adjusting pH and mobile phase composition, the wine is separated into three fractions of differing polarity. The content of each fraction can be monitored by UV-vis spectroscopy. When the experiment is combined with experiments involving HPLC or GC separations, students gain a greater appreciation for and understanding of the highly automated instrumental systems currently available. In addition, they learn about the chemistry of polyphenolic compounds, which are present in many foods and beverages and which are receiving much attention for their potentially beneficial health effects.
Supplement
A student handout, which includes background information with tables and structures, a materials and equipment list, instructions for carrying out the experiment, and references are provided.
*  Contents
*  Download
supp1710.pdf

supp1710.zip

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More Information
*  Citation
 Brenneman, Charles A.; Ebeler, Susan E. J. Chem. Educ. 1999 76 1710.
*  Keywords
 Public Understanding; Chromatography; Natural Products; polyphenols; Separation Science; Laboratory Instruction
*  History
 Created:
Last Updated:		 November 10, 1999
November 22, 2005
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