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  Home > JCE Print > Journal of Chemical Education > Issues > 1999  > January  >
In the Laboratory
The Preparation of a Fluorescent Dye: A Microscale TLC Experiment
Daniel S. Calimente, Shannon M. Strand, Shao-Chieh Chang, and David E. Lewis
Department of Chemistry & Biochemistry, South Dakota State University, Box 2202, Brookings, SD 57007-0896
Cover
January 1999
Vol. 76 No. 1
p. 82
Abstract
This paper describes an experiment that allows the instructor to introduce TLC as a technique for analyzing reaction mixtures by monitoring the formation of a fluorescent dye. The experiment also illustrates the effects of eluting solvent on elution order. The visual impact of the fluorescent compounds on visualization under long-wavelength (365 nm) ultraviolet light constitutes a major improvement over most other TLC experiments.
More Information
*  Citation
 Calimente, Daniel S.; Strand, Shannon M.; Chang, Shao-Chieh; Lewis, David E. J. Chem. Educ. 1999 76 82.
*  Keywords
 Organic Chemistry; Chromatography; Dyes; Luminescence; Microscale; Laboratory Instruction
*  History
 Created:
Last Updated:		 June 15, 1999
June 22, 2005
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