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  Home > JCE Print > Journal of Chemical Education > Issues > 1999  > June  >
In the Laboratory
Micellular Electrokinetic Capillary Chromatography in the Undergraduate Curriculum: Separation and Identification of the Amino Acid Residues in an Unknown Dipeptide Using FMOC Derivatization
Timothy G. Strein, James L. Poechmann, and Mark Prudenti
Department of Chemistry, Bucknell University, Lewisburg, PA 17837
Cover
June 1999
Vol. 76 No. 6
p. 820
Abstract
This manuscript describes our efforts to introduce biochemistry students to micellar electrokinetic capillary chromatography (MEKC), a mode of capillary electrophoresis that employs micelles in the operating buffer. Unlike free solution capillary electrophoresis, MEKC is capable of resolving both charged and uncharged analytes because the micellar pseudo stationary phase allows for the separation of uncharged species. The experiment described herein includes a comparison of MEKC, employing sodium dodecyl sulfate (SDS) as the micelle-forming species, with reverse-phase HPLC. Both methods are used to determine the amino acid residues in an unknown dipeptide. Advanced undergraduate chemistry, biochemistry, and biology majors perform this experiment in the Biochemical Methods course at Bucknell University. The students cleave the peptide bond, derivatize the resultant amino acids with 9-fluorenylmethyl chloroformate (FMOC), and separate the FMOCÐamino acid derivatives using HPLC and MEKC. This manuscript details the analytical procedures for the MEKC separation and presents typical student data obtained using this relatively new method.
More Information
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Citation
Strein, Timothy G.; Poechmann, James L.; Prudenti, Mark. J. Chem. Educ. 1999, 76, 820.
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Keywords
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History
Created:
Last Updated:
6/11/2008
6/13/2008
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