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  Home > JCE Print > Journal of Chemical Education > Issues > 1999  > May  >
In the Laboratory
Examining the Rate of Renaturation for Genomic DNA Isolated from E. coli: An Integrated Physical Biochemistry Laboratory
Nayhan Fancy and Andrew F. Mehl
Knox College, Department of Chemistry, Box K-7, Galesburg, IL 61401
Cover
May 1999
Vol. 76 No. 5
p. 646
Abstract
The second-order rate constant for the renaturation of DNA was determined for E. coli genomic DNA. The procedure involves the initial melting of the double-stranded DNA at high temperature and then an abrupt lowering of the temperature so that renaturation can occur. The absorbance at 260 nm is monitored during the entire procedure. This laboratory can be used as a project-based exercise in which students would initially have to purify the DNA and analyze the product for purity, and then carry out the kinetic measurements.
More Information
*  Citation
 Fancy, Nayhan; Mehl, Andrew F. J. Chem. Educ. 1999 76 646.
*  Keywords
 Biochemistry; Laboratory Instruction; Kinetics; deoxynucleic acid; DNA
*  History
 Created:
Last Updated:		 June 11, 1999
June 23, 2005
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