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  Home > JCE Print > Journal of Chemical Education > Issues > 2000  > August  >
In the Laboratory
An Inexpensive Gradient Maker for the Biochemistry Laboratory
William H. Flurkey
Department of Chemistry, Indiana State University, Terre Haute, IN 47809
Cover
August 2000
Vol. 77 No. 8
p. 1041
Abstract
A gradient maker for use in the biochemistry laboratory was constructed out of plastic graduated cylinders or other easily obtained plastic items. Assembly of the gradient makers requires little time or expense. Details on construction and operation are given. The gradient makers were used to elute proteins from ion-exchange columns, and they showed a linear increase in conductivity throughout the gradient volume. These items provide an inexpensive alternative to more expensive commercial gradient makers.
More Information
*  Citation
 Flurkey, Bill. J. Chem. Educ. 2000 77 1041.
*  Keywords
 Biochemistry; Chromatography; Laboratory Equipment / Apparatus; Proteins / Peptides
*  History
 Created:
Last Updated:		 July 5, 2000
April 15, 2005
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