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  Home > JCE Print > Journal of Chemical Education > Issues > 2000  > December  >
In the Laboratory
Topics in Chemical Instrumentation
Quantitative Analysis of Non-UV-Absorbing Cations in Soil Samples by High-Performance Capillary Electrophoresis. An Experiment for Undergraduate Instrumental Analysis Laboratory
Jason A. Gruenhagen, Dana Delaware, and Yinfa Ma
Division of Science, Truman State University, Kirksville, MO 63501

Cover
December 2000
Vol. 77 No. 12
p. 1613

Abstract
Cations such as Ca2+, Mg2+, K+, NH4+, Na+, Mn2+, and Zn2+ are very important in soil even though some of them are present in low concentrations. Plants, to grow well, must be planted in soil with cations in sufficient quantities and proper ratios. The number of cations and the variability of their concentrations in soils may make soil too complex for quantitative analysis by traditional methods. We describe a high-performance capillary electrophoresis (HPCE) experiment for quantitative analysis of cations in soil samples. The method is economical and fast, and is potentially applicable to normal soil analysis. Since HPCE is a useful and modern technique, it is necessary that undergraduate students in chemistry learn to use it. This experiment is designed as a one- or two-week laboratory experiment in Instrumental Analysis. It has been performed in the laboratory at Truman State University for two years, and students have obtained excellent results.
Supplement
The student handout and notes for the instructor are available.
*  Contents JCE2000p1613W.doc (Microsoft Word 2000, Windows)
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More Information
*  Citation
Gruenhagen, Jason A.; Delaware, Dana; Ma, Yinfa. J. Chem. Educ. 2000 77 1613.
*  Keywords
Analytical Chemistry; Electrophoresis; Environmental Chemistry; Instrumental Methods; Laboratory Instruction; Separation Science
*  History
Created:
Last Updated:
November 3, 2000
August 31, 2005
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