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  Home > JCE Print > Journal of Chemical Education > Issues > 2000  > December  >
In the Laboratory
Fluorescence Measurement of Pyrene Wall Adsorption and Pyrene Association with Humic Acids. An Experiment for Physical Chemistry or Instrumental Methods
Edward C. Shane, Miranda Price-Everett, and Tonya Hanson
Chemistry Department, Morningside College, Sioux City, IA 51106
Cover
December 2000
Vol. 77 No. 12
p. 1617
Abstract
We describe a two-part fluorescence experiment that measures (i) the surface adsorption of the polycyclic aromatic hydrocarbon (PAH) pyrene to Pyrex, quartz, and LDPE surfaces and (ii) the binding of pyrene to natural humic acids (HA). A model for surface adsorption is presented and used to measure wall adsorption constants for pyrene. Pyrene was found to have the strongest adsorption to LDPE and weaker adsorption to Pyrex and quartz. A fluorescence quenching method is described that allows measurement of the association of pyrene with HA while minimizing competition with pyrene wall adsorption. A model for PAH association with HA is presented and evaluated. Association constants are reported for the association of pyrene with three natural humic acids. The adaptability of the PAH-HA association experiment as a project laboratory is discussed.
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*  Citation
 Shane, Edward C.; Price-Everett, Miranda; Hanson, Tonya. J. Chem. Educ. 2000 77 1617.
*  Keywords
 Environmental Chemistry; Fluorescence Spectrometry; Instrumental Methods; Laboratory Instruction; Mechanisms; Physical Chemistry
*  History
 Created:
Last Updated:		 November 3, 2000
August 31, 2005
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