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2000
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December
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In the Laboratory
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ELISA and GC-MS as Teaching Tools in the Undergraduate Environmental Analytical Chemistry Laboratory
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Ruth I. Wilson, Dan T. Mathers, and Scott A. Mabury
Department of Chemistry, University of Toronto, Toronto, ON M5S 3H6, Canada
Greg M. Jorgensen
Department of Environmental Toxicology, University of California, Davis, CA 95615
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December 2000
Vol. 77 No. 12
p. 1619
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| Abstract |
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An undergraduate experiment for the analysis of potential water pollutants is described. Students are exposed to two complementary techniques, ELISA and GC-MS, for the analysis of a water sample containing atrazine, desethylatrazine, and simazine. Atrazine was chosen as the target analyte because of its wide usage in North America and its utility for students to predict environmental degradation products. The water sample is concentrated using solid-phase extraction for GC-MS, or diluted and analyzed using a competitive ELISA test kit for atrazine. The nature of the water sample is such that students generally find that ELISA gives an artificially high value for the concentration of atrazine. Students gain an appreciation for problems associated with measuring pollutants in the aqueous environment: sensitivity, accuracy, precision, and ease of analysis. This undergraduate laboratory provides an opportunity for students to learn several new analysis and sample preparation techniques and to critically evaluate these methods in terms of when they are most useful.
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| Supplement |
Student and teacher instructions are available.
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 Contents |
JCE2000p1619W.doc (Microsoft Word 97, Windows); JCE2000p1619W.cdr (Corel Draw 8.0, Windows)
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| More Information |
Citation |
Wilson, Ruth I.; Mathers, Dan T.; Mabury, Scott A.; Jorgensen, Greg M. J. Chem. Educ. 2000 77 1619.
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Keywords |
Analytical Chemistry; Chromatography; Environmental Chemistry; Laboratory Instruction; Mass Spectrometry
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History |
Created:
Last Updated: |
November 3, 2000
August 31, 2005
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December
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