Separation and Quantification of Preservatives Using Ion Pair HPLC and CZE: An Extended Investigation of Separation Mechanisms
Mary Boyce and Evadne Spickett
Faculty of Communications, Health and Science, Edith Cowan University, Mount Lawley Campus, Perth, Western Australia 6050
This paper describes the separation of preservatives in food and cosmetic creams by reversed-phase high-performance liquid chromatography (HPLC), ion pair HPLC, and capillary zone electrophoresis (CZE). The experiments described here are designed to promote understanding of the separation mechanisms involved and give students an opportunity to compare and evaluate the different techniques.
Supplement
The following supplemental material is available: instructor notes (chemical and equipment requirements and lab preparation, background information and references, typical set of student results, miscellaneous information and trouble-shooting); and the student experiment (background, procedure, references).
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