An experiment for instrumental analysis is described in which components of diet tonic water are determined using capillary electrophoresis. Separation of quinine, saccharin, and benzoate in pH 7 phosphate buffer, with phenol as internal standard, is accomplished in about 12 minutes. The equipment requirements are modest: UV detection on an unmodified column. One of the components, quinine, is quantitated using a four-point standard addition calibration curve.
Supplement
Supplemental material includes a student handout that contains a complete description of the experiment.
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