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  Home > JCE Print > Journal of Chemical Education > Issues > 2000  > November  >
In the Laboratory
A Biochemical Study of Noncovalent Forces in Proteins Using Phycocyanin from Spirulina
Barbara A. Heller and Yvonne M. Gindt
Department of Chemistry, University of Nebraska at Kearney, Kearney, NE 68849-1150
Cover
November 2000
Vol. 77 No. 11
p. 1458
Abstract
Protein tertiary structure is maintained by noncovalent forces. These forces are disrupted by simple environmental changes (increasing temperature, pH, or adding denaturants) but are not always simple to observe. This experiment, suitable for all levels of undergraduate biochemistry laboratories, uses absorption spectroscopy to monitor the denaturation of a pigmented protein. The protein, phycocyanin, contains a covalently attached pigment whose visible spectrum is sensitive to changes in protein environment. The experiment can be done in one or two 3-hour lab periods. The first session isolates the protein from cyanobacteria capsules and the second session studies the denaturation of the protein.
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Notes for the instructor and a student handout including background information, detailed instructions, and data sheet forms are available.
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*  Citation
 Heller, Barbara A.; Gindt, Yvonne M. J. Chem. Educ. 2000 77 1458.
*  Keywords
 Biochemistry; Intermolecular Forces; Proteins / Peptides; UV-Vis Spectroscopy
*  History
 Created:
Last Updated:		 October 6, 2000
August 31, 2005
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