JCE Online Journal of Chemical Education
 | Subscriptions  | Software Orders  | Support  | Contributors  | Advertisers  | 

JCE Print
   Current Issue
 Previous Issues
 Supplements
 Search JCE Index

JCE Digital Library
   DigiDemos
 QBank
 SymMath
 WebWare

JCE Software
   Latest Releases
 Software & Video
 Downloads
 Support

Only@JCE Online
   JCE Online Store
 JCE HS CLIC
 JCE Discussion Forums
   Biographical Snapshots
 ChemEd Resource Shelf
 Featured Molecules
 Hal's Picks
 Project Chemlab
 Reviewed WWW Sites
 "Web-Ed" Articles

About JCE
   Features
 Publications
 Operations
 Outreach
 Contact Us


  Home > JCE Print > Journal of Chemical Education > Issues > 2000  > November  >
In the Laboratory
Identification and Quantitation of Plasma Membrane Components: A Biochemical Experiment for Lipid Investigations
Susan Keys
Biology/Chemistry Department, Springfield College, Springfield, MA 01109-3797
Cover
November 2000
Vol. 77 No. 11
p. 1463
Abstract
In this biochemistry exercise, students isolate and investigate lipid components of erythrocyte membranes. Erythrocytes are separated from sheep's blood by centrifugation. Lipids are extracted using chloroform-methanol (2:1 by volume). Individual lipid components (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, sphingomyelin, and cholesterol) are separated and identified by thin-layer chromatography using chloroform-methanol-acetic acid-0.9% NaCl (75:45:3:1 by volume) as solvent. Nanomolar amounts of phospholipids are detected and quantified using a spectrophotometric phosphorus assay. Students learn to construct and use a standard concentration curve and test its accuracy.
Supplement
Student handout and instructor notes are available.
*  Contents JCE2000p1463W.wpd (Word Perfect, Windows)
*  Download
JCE2000p1463W.pdf

JCE2000p1463W.sit

JCE2000p1463W.zip
More Information
*  Citation
 Keys, Susan. J. Chem. Educ. 2000 77 1463.
*  Keywords
 Biochemistry; Chromatography; Laboratory Instruction; Lipids; Nanotechnology
*  History
 Created:
Last Updated:		 October 6, 2000
August 31, 2005
<< Previous Article Table of Contents Next Article >>
  Home > JCE Print > Journal of Chemical Education > Issues > 2000  > November  > Page 1463


Subscriptions
 Subscription Info
 Order Forms

JCE HS CLIC

Our Secondary School editors work hard to distill all the JCE materials to produce a fraction of particular interest to high school teachers. We call it CLIC.
 JCE HS CLIC

Contributions Welcome
JCE welcomes your submission
 Contributors' Corner

Advertisers
In recent years we have worked hard to better match our advertisers with our readers. When shopping for chemistry education materials, visit our advertisers' WWW sites first.
 Recent Advertisers

Be An Ambassador
Take JCE along on your outreach missions. Copies of the Journal, guest access to JCE Online, our publications catalog, and more are available for your participants.
 Outreach with JCE

Comments to
jceonline@chem.wisc.edu		 Copyright © Division of Chemical Education, Inc., American Chemical Society.
All rights reserved.