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  Home > JCE Print > Journal of Chemical Education > Issues > 2000  > October  >
In the Laboratory
Candies to Dye for: Cooperative, Open-Ended Student Activities to Promote Understanding of Electrophoretic Fractionation
Randall Emry
Southeast High School, 2930 S. 37th St., Lincoln, NE 68506

Robert D. Curtright
Northeast High School, 2635 N. 63rd St., Lincoln, NE 68507

Jonathan Wright and John Markwell
Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0664

Cover
October 2000
Vol. 77 No. 10
p. 1323
Abstract
A three-part series of laboratory activities is presented that allows students to learn why electrophoretic separations work and to manipulate the factors that influence the separation process. In the first two exercises, students perform cooperative separations of FD&C dyes from candies utilizing electrophoresis in two buffers of different pH. Students must use package information from one brand of candy and critical thinking to determine the identities of these dyes. Using structural formulas for the dyes the students draw conclusions regarding the effect of charge and molecular mass on separation. The final activity is an open-ended investigation into a student-formulated question. These activities integrate topics in biology and chemistry into activities that interest students and foster a real understanding of the tool of electrophoresis.
Supplement
Student handouts and instructor notes are available as supplemental material.
*  Contents JCE2000p1323Wfigures.doc (Microsoft Word 98, Macintosh), JCE2000p1323Wstudent.doc (Microsoft Word 98, Macintosh), JCE2000p1323Wteacher.doc (Microsoft Word 98, Macintosh), F0190878.pic (Photoshop PICT, Macintosh), F0290878.cdr (ChemDraw), F0390878.cdr (ChemDraw), F0490878.pic (Photoshop PICT, Macintosh)
*  Download
JCE2000p1323W.pdf

JCE2000p1323W.sit

JCE2000p1323W.zip
More Information
*  Citation
 Emry, Randall; Curtright, Robert D.; Wright, Jonathan; Markwell, John. J. Chem. Educ. 2000 77 1323.
*  Keywords
 Biochemistry; Dyes; Electrophoresis; Laboratory Instruction; Separation Science
*  History
 Created:
Last Updated:		 September 22, 2000
August 31, 2005
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