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  Home > JCE Print > Journal of Chemical Education > Issues > 2000  > April  >
In the Laboratory
Chemistry and Flatulence: An Introductory Enzyme Experiment
John R. Hardee, Tina M. Montgomery, and Wray H. Jones
Department of Chemistry, Henderson State University, Arkadelphia, AR 71999
Cover
April 2000
Vol. 77 No. 4
p. 498
Abstract
An inexpensive introductory-level enzyme experiment was developed using raffinose family sugars extracted from green split peas as a substrate and the enzymes alpha-galactosidase and sucrase found in Beano. The reaction studied was the hydrolysis of raffinose family sugars to galactose, glucose, and fructose, and the reaction rate was determined using a retail glucometer to measure the concentration of glucose. Results are given on the effect of substrate concentration, enzyme concentration, temperature, and heavy metals on enzyme activity.
More Information
*  Citation
 Hardee, John R.; Montgomery, Tina M.; Jones, Wray H. J. Chem. Educ. 2000 77 498.
*  Keywords
 Introductory / High School Chemistry; Nonmajor Courses; Enzymes; Food Science
*  History
 Created:
Last Updated:		 March 2, 2000
April 15, 2005
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