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  Home > JCE Print > Journal of Chemical Education > Issues > 2000  > February  >
In the Laboratory
Dipeptide Sequence Determination: Analyzing Phenylthiohydantoin Amino Acids by HPLC
Janice S. Barton, Chung-Fei Tang, and Steven S. Reed
Department of Chemistry, Washburn University, Topeka, KS 66621
Cover
February 2000
Vol. 77 No. 2
p. 268
Abstract
Amino acid composition and sequence determination, important techniques for characterizing peptides and proteins, are essential for predicting conformation and studying sequence alignment. This experiment presents improved, fundamental methods of sequence analysis for an upper-division biochemistry laboratory. Working in pairs, students use the Edman reagent to prepare phenylthiohydantoin derivatives of amino acids for determination of the sequence of an unknown dipeptide. With a single HPLC technique, students identify both the N-terminal amino acid and the composition of the dipeptide. This method yields good precision of retention times and allows use of a broad range of amino acids as components of the dipeptide. Students learn fundamental principles and techniques of sequence analysis and HPLC.
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*  Citation
 Barton, Janice S.; Tang, Chung-Fei; Reed, Steven S. J. Chem. Educ. 2000 77 268.
*  Keywords
 Biochemistry; Laboratory Instruction; Amino Acids; Chromatography
*  History
 Created:
Last Updated:		 January 5, 2000
August 31, 2005
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