The construction and use of a laser fluorimeter from a 635-nm red diode laser and an amplified photodiode detector are described. The low cost and monochromatic nature of diode lasers make them attractive as excitation sources for educational fluorescence experiments. Use of this type of fluorimeter is demonstrated by measuring fluorescence signals for various concentrations of Nile blue A dissolved in methanol; concentrations as low as 1 ppb are easily detected. The use of this instrument for monitoring the decomposition of a dye by an oxidizing agent is demonstrated by measuring the decay of fluorescence as a function of time for a 1 ppm Nile blue A solution after the addition of sodium hypochlorite.
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Tran, Yang; Whitten, J. E. J. Chem. Educ.2001 78 1093.
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