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  Home > JCE Print > Journal of Chemical Education > Issues > 2001  > August  >
In the Laboratory
Fluorescence Polarization as a Tool to Pinpoint Vesicle Thermal Phase Transitions

Gary A. Baker
Department of Chemistry, University at Buffalo, The State University of New York, Buffalo, NY 14260

Thomas A. Betts
Department of Physical Sciences, Kutztown University of Pennsylvania, Kutztown, PA 19530

Siddharth Pandey*
Department of Chemistry, New Mexico Institute of Mining and Technology, Socorro, NM 87801

Cover
August 2001
Vol. 78 No. 8
p. 1100
Abstract
Fluorescence polarization (FP) as a biophysical technique is simplistic in its approach and has potential for the direct determination of ligand-receptor binding and supramolecular associations. Here, students determine the gel-to-liquid transition temperature (Tm) in L-a-dimyristoylphosphatidylcholine (DMPC) model bilayer systems based on steady-state FP of a lipid-tailed fluorescent marker bound therein. First students are exposed to both organized media and emission polarization phenomena and then they apply these fundamentals to readily and accurately (±1-2 °C) determine Tm for a neat DMPC vesicular system.
Supplement
A data entry form for students and notes for the instructor on SUV preparation and equipment needs are available. Some additional tips for the instructor are also provided.
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*  Citation
 Baker, Gary A.; Betts, Thomas A.; Pandey, Siddharth. J. Chem. Educ. 2001 78 1100.
*  Keywords
 Fluorescence Spectrometry; Intermolecular Forces; Laboratory Instruction; Lipids; Phase Transitions / Diagrams
*  History
 Created:
Last Updated:		 July 10, 2001
August 31, 2005
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