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  Home > JCE Print > Journal of Chemical Education > Issues > 2001  > February  >
In the Laboratory
Determination of Flavonoids in Wine by High Performance Liquid Chromatography
Celeste da Queija, M. A. Queirós, and Ligia M. Rodrigues
Departamento de Química-IBQF, Universidade do Minho, Campus de Gualtar, 4700-320 Braga, Portugal
Cover
February 2001
Vol. 78 No. 2
p. 236
Abstract
The experiment presented is an application of HPLC to the analysis of flavonoids in wines, designed for students of instrumental methods. It is done in two successive 4-hour laboratory sessions. While the hydrolysis of the wines is in progress, the students prepare the calibration curves with standard solutions of flavonoids and calculate the regression lines and correlation coefficients. During the second session they analyze the hydrolyzed wine samples and calculate the concentrations of the flavonoids using the calibration curves obtained earlier. This laboratory work is very attractive to students because they deal with a common daily product whose components are reported to have preventive and therapeutic effects. Furthermore, students can execute preparative work and apply a more elaborate technique that is nowadays an indispensable tool in instrumental analysis.
More Information
*  Citation
 da Queija, Celeste; Queirós, M. A.; Rodrigues, Ligia M. J. Chem. Educ. 2001 78 236.
*  Keywords
 Chromatography; Instrumental Methods; Laboratory Instruction; Qualitative Analysis; Quantitative Analysis; Wines/Flavonoids
*  History
 Created:
Last Updated:		 December 22, 2000
August 31, 2005
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