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2001
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In the Laboratory
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UV-Visible First-Derivative Spectrophotometry Applied to an Analysis of a Vitamin Mixture
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F. Aberásturi, A. I. Jiménez, F. Jiménez, and J. J. Arias
Departamento de Química Analítica, Nutrición y Bromatología, Universidad de La Laguna, Facultad de Química, E-38071 La Laguna, Tenerife, Spain
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June 2001
Vol. 78 No. 6
p. 793
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| Abstract |
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A simple new experiment that uses UV-vis spectrophotometry to introduce undergraduate chemistry students to multicomponent analysis is presented and a method for the simultaneous determination of three vitamins using derivative spectrophotometry (zero-crossing method) is described. The methodology is simple and easy to apply and allows the determination of folic acid, pyridoxine, and thiamine over the concentration ranges 1.02-14.28, 1.00-16.00, and 6.00-20.00 mg mL-1, respectively. The resulting errors were nearly always less than 5%.
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| More Information |
 Citation |
Aberásturi, F.; Jiménez, A. I.; Jiménez, F.; Arias, J. J. J. Chem. Educ. 2001 78 793.
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Keywords |
Demonstrations; Laboratory Instruction; Undergraduate Research; UV-Vis Spectroscopy; Vitamins
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History |
Created:
Last Updated: |
May 8, 2001
August 31, 2005
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| Home > JCE Print > Journal of Chemical Education > Issues >
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