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  Home > JCE Print > Journal of Chemical Education > Issues > 2001  > March  >
In the Laboratory
What Factors Affect the Separation of Substances Using Thin-Layer Chromatography? An Undergraduate Experiment
John J. Nash, Jeanne A. Meyer, and Barbara Everson
Department of Chemistry, Purdue University, West Lafayette, IN 47907
Cover
March 2001
Vol. 78 No. 3
p. 364
Abstract
Rf values in thin-layer chromatography (TLC) depend strongly on solvent saturation of the atmosphere above the liquid in the TLC developing chamber. Although there are a multitude of experiments in the literature that describe the use of TLC for the analysis of a mixture, we were unable to find one in which the student discovers the importance of maintaining the solvent atmosphere during development of a TLC plate. The experiment reported here illustrates the potentially dramatic effects on TLC Rf values of not equilibrating the solvent atmosphere during development.
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*  Contents JCE2001p0364.wpd (Word Perfect)
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*  Citation
 Nash, John J.; Meyer, Jeanne A.; Everson, Barbara. J. Chem. Educ. 2001 78 364.
*  Keywords
 Chromatography; General Chemistry; Laboratory Instruction; Organic Chemistry
*  History
 Created:
Last Updated:		 February 6, 2001
August 31, 2005
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