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  Home > JCE Print > Journal of Chemical Education > Issues > 2001  > September  >
In the Laboratory
Thin-Layer Electrophoresis
Tom Lyons Fisher and I. David Reingold
Department of Chemistry, Juniata College, Huntingdon, PA 16652-2119

Tara Leslie Fitzsimmons
Juniata College Science Outreach Project, Huntingdon, PA 16652-2119
Cover
September 2001
Vol. 78 No. 9
p. 1241
Abstract
An inexpensive apparatus for thin-layer electrophoresis is utilized to separate amino acids. This method is especially useful for illustrating the principle of molecular charge to students at the high school and undergraduate levels. Construction of the apparatus and typical experiments are described. Practical lab applications are suggested, and labs appropriate for high school and undergraduate students are included in the supplemental materials.
Supplement
The following supplemental material for this article is available: an experiment separating amino acid mixtures that is appropriate for a high school lab, a simulation on mislabeled amino acids that is appropriate for an undergraduate lab, a chart of amino acids comparing charge and pH, a parts list, apparatus construction hints, and CAS registry numbers.
*  Contents JCE2001p1241W.DOC (MS Word)
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*  Citation
 Fisher, Tom Lyons; Fitzsimmons, Tara Leslie; Reingold, I. David. J. Chem. Educ. 2001 78 1241.
*  Keywords
 Amino Acids; Electrophoresis; Laboratory Equipment / Apparatus; Laboratory Instruction
*  History
 Created:
Last Updated:		 August 14, 2001
August 31, 2005
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