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  Home > JCE Print > Journal of Chemical Education > Issues > 2001  > September  >
In the Laboratory
Advanced Chemistry Classroom and Laboratory
Circular Dichroism of Globular Proteins
Brenda A. Bondesen and Merlyn D. Schuh
Department of Chemistry, Davidson College, Davidson, NC 28036
Cover
September 2001
Vol. 78 No. 9
p. 1244
Abstract
This paper describes experiments that teach junior/senior undergraduate students about some of the uses of circular dichroism in characterizing several aspects of globular protein structure. The four experiments are (i) qualitative comparison of CD spectra for globular proteins with different amounts of secondary and tertiary structures, (ii) characterization of secondary and tertiary structure of the A state of cytochrome c, which is believed to resemble the molten globule, (iii) kinetics of lysozyme denaturation, and (iv) helicogenesis of concanavalin A, whereby the predominantly b-sheet protein structure is converted into mostly a-helix. These experiments have the pedagogical advantage of giving students experience with a spectroscopic method that provides more important details about protein structures than is provided by most routine spectroscopic methods.
Supplement
A student handout containing background information and instructions for the experiment and a list of questions (with answers) is available.
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*  Citation
 Bondesen, Brenda A.; Schuh, Merlyn D. J. Chem. Educ. 2001 78 1244.
*  Keywords
 Biochemistry; Biophysical Chemistry; Circular Dichroism Spectroscopy; Kinetics; Laboratory Instruction; Proteins / Peptides
*  History
 Created:
Last Updated:		 August 14, 2001
August 31, 2005
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