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2001
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September
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In the Laboratory
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Advanced Chemistry Classroom and Laboratory
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NMR of a Phospholipid: Modules for Advanced Laboratory Courses
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Holly C. Gaede
Department of Chemistry, Ursinus College, Collegeville, PA 19426
Ruth E. Stark
Department of Chemistry, CUNY, College of Staten Island, Staten Island, NY 10314
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September 2001
Vol. 78 No. 9
p. 1248
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| Abstract |
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A laboratory project is described that builds upon the NMR experience undergraduates receive in organic chemistry with a battery of NMR experiments that investigate egg phosphatidylcholine (egg PC). This material, often labeled in health food stores as lecithin, is a major constituent of mammalian cell membranes. The NMR experiments may be used to make resonance assignments, to study molecular organization in model membranes, to test the effects of instrumental parameters, and to investigate the physics of nuclear spin systems. A suite of modular NMR exercises is described, so that the instructor may tailor the laboratory sessions to biochemistry, instrumental analysis, or physical chemistry. The experiments include solution-state one-dimensional (1D) 1H, 13C, and 31P experiments; two-dimensional (2D) TOtal Correlated SpectroscopY (TOCSY); and the spectral editing technique of Distortionless Enhancement by Polarization Transfer (DEPT). To demonstrate the differences between solution and solid-state NMR spectroscopy and instrumentation, a second set of experiments generates 1H, 13C, and 31P spectra of egg PC dispersed in aqueous solution, under both static and magic-angle spinning conditions.
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| Supplement |
A detailed student handout including questions, notes and answers for the instructor, and additional sample NMR spectra are available.
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 Contents |
JCE2001p1248W.doc (MS Word)
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| Download |
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| More Information |
Citation |
Gaede, Holly C.; Stark, Ruth E. J. Chem. Educ. 2001 78 1248.
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Keywords |
Biophysical Chemistry; Instrumental Methods; Laboratory Instruction; Lipids; Molecular Properties / Structure; NMR Spectrometry; Solid-State Chemistry
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History |
Created:
Last Updated: |
August 14, 2001
August 31, 2005
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| Home > JCE Print > Journal of Chemical Education > Issues >
2001
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September
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1248
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